CLN3p Impacts Galactosylceramide Transport, Raft Morphology, and Lipid Content

Lipid Raft Microdomains

Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggestin...

p27kip1 controls cell morphology and motility by regulating microtubule-dependent lipid raft recycling.

p27(kip1) (p27) is an inhibitor of cyclin/cyclin-dependent kinase complexes, whose nuclear loss indicates a poor prognosis in various solid tumors. When located in the cytoplasm, p27 binds Op18/stathmin (stathmin), a microtubule (MT)-destabilizing protein, and restrains its activity. This leads to MT stabilization, which negatively affects cell migration. Here, we demonstrate that this p27 func...

Lipid Raft-Associated GTPase Signaling

Lipid raft idea is floated

T he introduction of a fl uorescent lipid probe, specifi cally fl uorescent ceramide, in the mid-1980s (Lipsky and Pagano, 1983; Lipsky and Pagano, 1985) gave Gerrit van Meer and Kai Simons just the tool they needed to attack a nagging question. Lipid locations For a decade, it had been known that the apical and basolateral membranes of epithelial cells had different lipid compositions (Kawai, ...

Lipid Raft in Cardiac Health and Disease

Lipid rafts are sphingolipid and cholesterol rich micro-domains of the plasma membrane that coordinate and regulate varieties of signaling processes. Lipid rafts are also present in cardiac myocytes and are enriched in signaling molecules and ion channel regulatory proteins. Lipid rafts are receiving increasing attention as cellular organelles contributing to the pathogenesis of several structu...